Frontiers | Chemical Proteomic Analysis of Serine Hydrolase Evaluation of endocannabinoid hydrolase activity in native tissue may provide insight in the role of the ECS in physiological and disease processes. Interestingly, endocannabinoid levels are elevated during neurodegeneration and neuroinflammation (van der Stelt and Di Marzo, 2005; Nomura et al., 2011; Chiurchiù et al., 2017). Ablation of PM20D1 reveals N-acyl amino acid control of (2), and endocannabinoid hydrolase inhibitors are in clinical de-velopment forneurological disorders(3). Other,lesscharacterized families of bioactive lipids have also been detected in mammalian tissues. One such family is the N-acyl amino acids (NAAs). NAAs are a composed of a fatty acyl chain linked to an amino acid by an amide bond (4 Chemical Proteomics Maps Brain Region Specific Activity of Chemical Proteomics Maps Brain Region Specific Activity of Endocannabinoid Hydrolases Cannabis and Cannabinoid Biology in Stroke | Stroke

Monoacylglycerol Lipase Inhibition in Human and Rodent Systems

Development of a Multiplexed Activity-Based Protein Profiling Assay to Evaluate Activity of Endocannabinoid Hydrolase Inhibitors Antonius P. A. Janssen,† Daan van der Vliet,† Alexander T. Bakker,† Ming Jiang,† The Endogenous Cannabinoid System: A Budding Source of Targets A great need exists for the development of new medications to treat pain resulting from various disease states and types of injury. Given that the endogenous cannabinoid (that is, endocannabinoid EVALUATION OF NHS CARBAMATES AS A HIGHLY POTENT AND SELECTIVE

endocannabinoid hydrolase (FAAH, MAGL, ABHD6/12) over other serine hydrolases (SHs) of mouse brain membrane proteomes (see subsection 5.3.12).

Inhibition of the endocannabinoid catabolic enzymes, monoacylglycerol lipase (MAGL) or fatty acid amide hydrolase (FAAH) attenuates naloxone-precipitated opioid withdrawal signs in mice via Membrane lipids are key modulators of the Membrane lipids are key-modulators of the endocannabinoid-hydrolase FAAH Enrico Dainese 1,2¥* , Gianni De Fabritiis 3¥ , Annalaura Sabatucci 1 , Sergio Oddi 4 ,2 , Clotilde Beatrice ASSOCIATE EDITOR: ERIC L. BARKER Chemical Probes of Endocannabinoid Hydrolase Inhibitors 851. higher than anandamide levels, although the relevance of this difference on their signaling actions is unclear, especially considering that their basal extracellular levels, as measured by in vivo microdialysis, a Chemical Proteomics Maps Brain Region Specific Activity of The biosynthetic and catabolic enzymes of the endocannabinoids tightly regulate endocannabinoid-mediated activation of the cannabinoid CB1 receptor. Monitoring the activities of these endocannabinoid hydrolases in different brain regions is, therefore, key to gaining insight into spatiotemporal control of CB1 receptor-mediated physiology.

11 Sep 2012 Generation of t ransient endocannabinoid hydrolase-overexpressing The fluorescent glycerol assay for endocannabinoid hydrolase activity.

Sigma-Aldrich offers abstracts and full-text articles by [Jacqueline L Blankman, Benjamin F Cravatt]. Monoacylglycerol Lipase Inhibition in Human and Rodent Systems Determination of Inhibitor Potency by Substrate Assays. 2-AG hydrolysis activity in human brain cortex membrane homogenates (20 μg in 200 μl PBS) treated with ABD-1970 or DMSO vehicle (30 minutes at 37°C) was assessed using an MS-based substrate assay, essentially as previously described (Blankman et al., 2007). Attenuation of serotonin-induced itch responses by inhibition of Itch and pain are two irritating sensations sharing a lot in common. Considering the antinociceptive effects of blockade of endocannabinoid degrading enzymes in pain states, we attempted to reduce scratching behavior by endocannabinoid modulation … An Activity‐Based Imaging Probe for the Integral Membrane Department of Chemical Physiology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037(USA) These authors contributed equally to this work.